| 书目名称 | Gigaseal Formation in Patch Clamping |
| 副标题 | With Applications of |
| 编辑 | Majid Malboubi,Kyle Jiang |
| 视频video | |
| 概述 | Explains the mechanisms of gigaseal formation to the latest level of discoveries.Provides practical techniques, which enable frequent formation of high resistance seals.Shares authors´ recent practice |
| 丛书名称 | SpringerBriefs in Applied Sciences and Technology |
| 图书封面 |  |
| 描述 | .This book presents an investigation of gigaseal formation using micro/nanotechnology. The aims of the book are twofold. First, it explains the mechanisms of gigaseal formation using the latest discoveries. Second, it provides practical techniques for frequent formation of high resistance seals. The formation of a high-resistance electrical seal, also known as a gigaseal, between a cell membrane and a glass micropipette tip is essential in patch-clamp experiments. Even though four decades have passed since the introduction of the patch-clamping technique by Neher and Sakmann, gigaseal formation remains an obstacle in developing the high-throughput ion channel screening systems required by the pharmaceutical industry. Here the authors share their latest methods for achieving gigaseal formation and describe techniques that are highly desirable at both research and industrial levels. Nanotechnology has been found to be a powerful tool for studying and modifying glass micropipettes and in tackling the problem of gigaseal formation.. |
| 出版日期 | Book 2014 |
| 关键词 | Gigaseal Formation using micropipettes; High Resistance Seals; High throughput screening; Hydrophilicit |
| 版次 | 1 |
| doi | https://doi.org/10.1007/978-3-642-39128-6 |
| isbn_softcover | 978-3-642-39127-9 |
| isbn_ebook | 978-3-642-39128-6Series ISSN 2191-530X Series E-ISSN 2191-5318 |
| issn_series | 2191-530X |
| copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer-Verlag GmbH, DE |
发表于 2025-3-21 16:59:46
