Titlebook: Germline Stem Cells; Methods and Protocol Michael Buszczak Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under e

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Identification of Germline Stem Cells in Zebrafish,ethod is presented here for identifying germline stem cells in the ovary and testis using a combined protocol for whole-mount fluorescent RNA in situ hybridization to detect . mRNA and immunofluorescence to detect the pan-germ cell marker Vasa.

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Maintenance of Human Primordial Germ Cell-Like Cells in a Long-Term Culture System,vitro models of gametogenesis have allowed the field to generate human primordial germ cell-like cells (hPGCLCs). In this chapter, we will review the generation of hPGCLCs using the incipient mesoderm-like cell (iMeLC) protocol and the subsequent expansion of hPGCLCs in a long-term culture system.

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https://doi.org/10.1007/978-3-658-27172-5at, in the naked mole-rat (.) ovary, there is mitotic expansion of the PGCs, and the initiation of the meiotic program occurs postnatally. In this chapter, we present a comprehensive collection of protocols that permit the analysis of naked mole-rat germ cells, from PGCs to oocytes, in meiotic prophase I, using in vivo and in vitro approaches.

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David Jou,José Casas-Vázquez,Georgy Lebone for the isolation or enrichment of primordial germ cells (from 9.5 days . (.) to 18.5 .) by flow cytometry (Subheading .) or magnetic sorting (Subheading .), followed by feeder-free primary germ cell culture (Subheading .).

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Isolation and Culture Techniques for Fetal Mouse Germ Cells,e for the isolation or enrichment of primordial germ cells (from 9.5 days . (.) to 18.5 .) by flow cytometry (Subheading .) or magnetic sorting (Subheading .), followed by feeder-free primary germ cell culture (Subheading .).

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Analysis of the , Germline Stem Cell Pool,cell state to a differentiated state. The germline tissue is organized in an assembly line with the germline stem cell (GSC) pool at one end and differentiated gametes at the other. A simple mesenchymal niche caps the GSC pool and maintains GSCs in an undifferentiated state by signaling through the

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