Titlebook: Germline Stem Cells; Methods and Protocol Michael Buszczak Book 2023Latest edition The Editor(s) (if applicable) and The Author(s), under e

TEN

浮夸

Hyperplasia

Targeting Endogenous Loci That Function in , Germline Stem Cells,e other genetic manipulations is useful for studying germline stem cell biology. In this chapter, we will describe a method to use CRISPR-Cas9 genome editing technology to make knock-out and knock-in flies. We will cover everything from guideRNA (gRNA) and donor plasmid design and cloning to screeni

Choreography

Genetics

Exposure

Spermatogonial Dedifferentiation into Germline Stem Cells in , Testes,on as a conserved mechanism for replenishing stem cell pools after their loss, thereby maintaining tissue homeostasis. The testis of the fruit fly . offers a simple but powerful system to study dedifferentiation, the process by which differentiating spermatogonia can revert their fate to become full

雄伟

施舍

1FAWN

Heretical

A Roadmap for Three-Dimensional Analysis of the Intact Mouse Ovary,n of the dynamic changes occurring at the single-cell level. Here we describe methods for whole-mount immunofluorescence, clearing, imaging, and analysis of whole ovarian tissue in 3D throughout murine development and aging.