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Titlebook: Germline Development in the Zebrafish; Methods and Protocol Roland Dosch Book 2021 Springer Science+Business Media, LLC, part of Springer N

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Export Credit Insurance and Guaranteesng of this complicated process is utilization of severely sex-biased families or groups. Here, we describe a method for partial depletion of primordial germ cells (PGCs) that leads to eventual masculinization of zebrafish. The technique is based on injecting early embryos with diluted morpholino oli
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The export and shipping office,re maternal and paternal genomes until embryo cryopreservation is achieved. However, the number of these cells in an embryo is very low limiting their potential application in cryopreservation and surrogate production. However, it was assumed that the induction of fish PGCs in vitro is not possible
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Export beruflicher Aus- und Weiterbildungrvation minimizes expenses for animal and facility maintenance such as personnel, water, power, and space. It extends the time offspring can be produced from individual organisms, reduces the need to maintain live populations, provides flexibility for planning future experiments and research project
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,Einführung in die Problemstellung, the same individual over time. Optical-based imaging methods can hardly be applied on the adult zebrafish, due to their limited transparency. In this chapter, we will demonstrate the application of micro computer tomography (CT) imaging for in vivo 3D imaging of the gonads in adult zebrafish. We ex
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,Die wichtigsten Fachausdrücke des Verkehrs,s of oogenesis involve the coordination of simultaneous and tightly sequential processes across cellular compartments and between sister cells. To fully understand the mechanistic framework of these coordinated processes, cellular and morphological analysis in high temporal resolution is required. H
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https://doi.org/10.1007/978-3-322-82854-5specialized follicle cell, called the micropylar cell (MC). Very little is known about the mechanisms underlying the specification and differentiation of the MC, a unique cell among hundreds that compose the follicle cell layer. The Hippo pathway effector Taz is essential for this process and is the
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