Titlebook: Germline Development in the Zebrafish; Methods and Protocol Roland Dosch Book 2021 Springer Science+Business Media, LLC, part of Springer N

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Observation of Medaka Larval Gonads by Immunohistochemistry and Confocal Laser Microscopy,ning the gonad just beneath the coverslips is essential to obtain high-resolution images at a level where cellular components of germ cells, such as germ plasm and nuclear structures, can be clearly observed using an oil immersion objective lens.

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Explosive Welding, Forming and Compactionay to reveal the expression of individual genes in the two compartments of the follicle. This chapter introduces a method for mechanic separation of the oocyte and follicle layer at full-grown stage for expression analysis. Since fish have similar follicle structure, this method may also be used in other species as well.

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Separation of Oocyte and Follicle Layer for Gene Expression Analysis in Zebrafish,ay to reveal the expression of individual genes in the two compartments of the follicle. This chapter introduces a method for mechanic separation of the oocyte and follicle layer at full-grown stage for expression analysis. Since fish have similar follicle structure, this method may also be used in other species as well.

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Roland DoschIncludes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts

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