Titlebook: Germ Cell Protocols; Volume 2: Molecular Heide Schatten Book 2004 Humana Press 2004

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Dual-Channel Confocal Ratioing of Calcium Dynamics in Living Eggs and Oocytes,ction with fluorescence microscopy to track calcium dynamics during fertilization. The relative benefits and disadvantages of aequorin vs calcium-sensitive probes have been discussed previously (.), and both types of calcium indicators remain widely employed in imaging analyses that involves conventional microscopy.

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Book 2004on of transgenic technologies and nuclear cloning. Basic knowledge and te- niques developed for lower vertebrate and invertebrate systems have facilitated the study of higher vertebrates, including humans. Many experiments that have first been performed on lower vertebrates provided the tools and st

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https://doi.org/10.1007/978-3-642-69906-1get mRNA (.–7). Consequently the animals display a phenotype that results from the depletion of the target protein. The RNAi phenotype can reach the severity of a null mutant (.). Although, generally RNAi is far more effective than antisense RNA (.,.) the effectiveness of a specific RNAi experiment depends on the target gene.

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