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Titlebook: Germ Cell Protocols; Volume 2: Molecular Heide Schatten Book 2004 Humana Press 2004

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Activity of the Germline-Specific ,-GFP Transgene in Normal and Clone Mouse Embryos,toside) to produce a blue color reaction. The green fluorescent protein (GFP) from the jellyfish . gives a natural green fluorescence that can be viewed under blue light excitation without processing the biological substrate for any chemical/enzymatic reaction. GFP is a convenient reporter for several reasons:
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In Vitro Fertilization and Embryo Transfer in Felids,omyelinosis C. The close phylogenetic relationship, and the fact that many analogous genetic disorders have been identified and characterized, are important factors that demonstrate the advantages of domestic cats as biomedical research models for human disease when compared to other laboratory animals.
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Activity of the Germline-Specific ,-GFP Transgene in Normal and Clone Mouse Embryos, reporter gene to tag the gene either in a construct (transgenesis) or in the genome (homologous recombination). Transgenes have been generated using different regions of the . gene promoter to drive expression of either lacZ or GFP, which allows the activity of the . promoter to be followed in livi
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Differentiating Embryonic Stem Cells into Embryoid Bodies,tained in a pluripotent state for indefinite periods of time in the presence of the leukemia inhibitory factor (LIF) or in coculture with mouse embryonic fibroblasts (MEFs). When cultured in the absence of LIF, ES cells differentiate spontaneously, forming three-dimensional (3D) aggregates called .
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