Titlebook: Germ Cell Development; Methods and Protocol Marco Barchi,Massimo De Felici Book 2024 The Editor(s) (if applicable) and The Author(s), under

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Demeter and Kore in Mississippi,ular levels. In the present chapter, I focus on immunomagnetic cell sorting (MACS) and fluorescence-activated cell sorting (FACS) methods used in my laboratory for the purification of mouse PGCs from 10.5 to 12.5 dpc embryos before their differentiation in oogonia/oocytes in female and prospermatogonia in male.

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https://doi.org/10.1007/978-3-030-44826-4ells, the epithelial cell fraction can be seeded in the macropores to resemble the in vivo testicular architecture. Moreover, macropores can serve as a delimitation for all testicular cells to reorganize and improve the supply of nutrients to cells through the 3D constructs.

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Purification and Transfection Methods of Chicken Primordial Germ Cellsdevelopmental stages. Here, we describe in detail a method based on the Percoll gradient, routinely used in our laboratory, allowing us to obtain from blood and gonad anlages significant numbers of viable PGCs which can be successfully cultured or efficiently genetically modified.

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