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Titlebook: Germ Cell Development; Methods and Protocol Marco Barchi,Massimo De Felici Book 2024 The Editor(s) (if applicable) and The Author(s), under

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书目名称Germ Cell Development
副标题Methods and Protocol
编辑Marco Barchi,Massimo De Felici
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Germ Cell Development; Methods and Protocol Marco Barchi,Massimo De Felici Book 2024 The Editor(s) (if applicable) and The Author(s), under
描述This detailed volume explores techniques for the isolation, purification, and establishment of in vitro germ cell (GS) systems at different stages of development in a variety of organisms, including chickens, mice, rats, and humans. The book additionally describes cutting-edge analytic and informatic tools to study germ cell development at the single cell level and meiotic recombination. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Germ Cell Development: Methods and Protocols. provides new insight into the understanding of basic biological aspects of germ cell biology, as well as the opportunity for in vitro manipulation of germ cells for toxicology studies and for genetic intervention..
出版日期Book 2024
关键词In vitro GC systems; Primordial germ cells (PGCs); Animal models; Cytological methods; Genetic informati
版次1
doihttps://doi.org/10.1007/978-1-0716-3698-5
isbn_softcover978-1-0716-3700-5
isbn_ebook978-1-0716-3698-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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Germ Cell Development978-1-0716-3698-5Series ISSN 1064-3745 Series E-ISSN 1940-6029
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Demeter and Kore in Mississippi,c information to subsequent generations. Studies aimed to unravel PGC development at molecular level in mammals can be traced at the early 1980s and were hampered by the difficulty in obtaining both sufficient quantities and purity of PGCs. For many laboratories, the isolation and purification metho
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Empörung, Wahrhaftigkeit und Common Sensepassed to the next generations. PGCs are found in different locations and variable numbers in the chick embryo, as in other species, depending on the developmental stages. Here, we describe in detail a method based on the Percoll gradient, routinely used in our laboratory, allowing us to obtain from
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https://doi.org/10.1007/978-1-349-16913-9s been used for many years to sub-divide these cells based on their DNA content. This approach provides an efficient way to obtain broad categories of male germ cells: pre-meiotic spermatogonia, meiotic spermatocytes and post-meiotic spermatids. The addition of a red filter for Hoechst staining enab
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