Titlebook: Genotype Phenotype Coupling; Methods and Protocol Stefan Zielonka,Simon Krah Book 2023Latest edition The Editor(s) (if applicable) and The

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Die Scheibe gleicher Festigkeit,thods for characterizing kinase–substrate interactions are limited by their inherently low throughput and the heterogeneity of samples analyzed. Recent advances in yeast surface display techniques provide new opportunities for studying individual kinase–substrate interactions in a stimulus-independe

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https://doi.org/10.1007/978-3-642-91141-5pproved in 2018, a major drawback that remains is the time-consuming reformatting of monoclonal antibody (mAb) candidates. By using a Golden Gate cloning (GGC)-dependent workflow, the bulk transfer of genetic information can be performed from antibody fragments displayed on yeast cells to a bidirect

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https://doi.org/10.1007/978-3-662-42546-6e recombinant antibody library approaches is ongoing, the major source of antibody-secreting cells (ASCs) remains to be primary B cells of mostly rodent origin. As fainting viability and secretion rates can lead to false-negative screening results, careful preparation of these cells is an essential

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https://doi.org/10.1007/978-3-7091-9980-0biotherapeutics. Besides random integration methods, targeted gene integration approaches have emerged as promising tools over the last few years. In addition to reducing heterogeneity within a pool of recombinant transfectants, this process can also facilitate shorter timelines of the current cell

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https://doi.org/10.1007/978-1-0716-3279-6Antibodies; Natively paired antibody libraries; Single cell technologies; Therapeutics; Antibody enginee

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978-1-0716-3281-9The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines

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