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Titlebook: Genotype Phenotype Coupling; Methods and Protocol Stefan Zielonka,Simon Krah Book 2023Latest edition The Editor(s) (if applicable) and The

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书目名称Genotype Phenotype Coupling
副标题Methods and Protocol
编辑Stefan Zielonka,Simon Krah
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Genotype Phenotype Coupling; Methods and Protocol Stefan Zielonka,Simon Krah Book 2023Latest edition The Editor(s) (if applicable) and The
描述This detailed new edition broadens the scope of the first edition by moving beyond classical display technologies. This book explores methodologies for the generation of natively paired antibody libraries, single cell technologies, alternative scaffolds, and in silico antibody sequence assessments are described. The application of these methods may allow for a generation of improved therapeutics and diagnostic reagents in a shorter time frame. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Genotype Phenotype Coupling: Methods and Protocols, Second Edition. serves as an ideal guide for researchers seeking to expand their knowledge of antibody-based therapeutics..
出版日期Book 2023Latest edition
关键词Antibodies; Natively paired antibody libraries; Single cell technologies; Therapeutics; Antibody enginee
版次2
doihttps://doi.org/10.1007/978-1-0716-3279-6
isbn_softcover978-1-0716-3281-9
isbn_ebook978-1-0716-3279-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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Die Berechnung der Marktnachfragellows for the accumulation of some contaminant clones into the selected phage pool and, consequently, each clone requires individual screening to verify its actual specificity. This step is time-consuming, independently on the chosen method, and relies on the availability of reliable reagents. Since
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Die Berechnung der Scheibenkolben,ogies for the discovery of therapeutic and diagnostic antibodies. Besides the importance of selection strategy, one key component of the successful isolation of highly specific recombinant antibodies is the construction of high-quality phage display libraries. However, previous cloning protocols rel
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https://doi.org/10.1007/978-3-662-30329-0or antibody fragment that specifically binds a target present on the surface of a tumor cell. Suitable antigens that can be used for immunotherapy are ideally tumor-specific or tumor-associated and stably expressed on the tumor cell. The identification of new target structures to further optimize im
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,Stabilität, Ring- und Längsmomente, VHH and shark variable new antigen receptor domains (VNAR). Bovines also contain a unique “ultralong CDRH3” with a conserved structural motif, comprising a knob domain and β-stalk. When removed from the antibody scaffold, either the entire ultralong CDRH3 or the knob domain alone, is typically capa
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