Titlebook: Genotype Phenotype Coupling; Methods and Protocol Stefan Zielonka,Simon Krah Book 2020 Springer Science+Business Media, LLC, part of Spring

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https://doi.org/10.1007/978-3-0348-5883-0ake them useful for many applications. In 2007, our group paved the way for using archaeal Sul7d proteins for the design of artificial affinity ligands, so-called Affitins. For many years, Sac7d and Sso7d have been used as molecular basis to obtain binders for various targets. Recently, we character

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https://doi.org/10.1007/978-3-642-45996-2nds of peptides and proteins. In this method, the puromycin-linker is a key molecule to realize smart genotype–phenotype coupling. A recently improved puromycin-linker and its use were explained in detail for the in vitro selection of peptides and proteins using the cDNA display method.

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Zur Anatomie der Belemnitentiere, here experimental procedures for a cDNA display IVE to select peptide aptamers from a scaffold-based random peptide library. A three-finger (3-F) peptide library is exemplified, which has been shown its pluripotency to various target molecules. Peptide scaffolds including 3-F are refined through ev

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