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Titlebook: Genotype Phenotype Coupling; Methods and Protocol Stefan Zielonka,Simon Krah Book 2020 Springer Science+Business Media, LLC, part of Spring

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书目名称Genotype Phenotype Coupling
副标题Methods and Protocol
编辑Stefan Zielonka,Simon Krah
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes an implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Genotype Phenotype Coupling; Methods and Protocol Stefan Zielonka,Simon Krah Book 2020 Springer Science+Business Media, LLC, part of Spring
描述.This volume aims at providing state-of-the-art protocols detailing ribosome display, cDNA display, phage display, yeast surface display, and mammalian display. Chapters guide readers through methods and protocols on in vitro methods over prokaryotic display systems, lower eukaryotes, and mammalian cells. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of all necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.. .Authoritative and cutting-edge, .Genotype Phenotype Coupling .aims to provide an overview of current technologies in this exciting and continuously evolving field. . .
出版日期Book 2020
关键词phage-assisted Continuous Evolution (PACE); Phage Display; SELEX; genotype phenotype coupling; Aptamers
版次1
doihttps://doi.org/10.1007/978-1-4939-9853-1
isbn_softcover978-1-4939-9855-5
isbn_ebook978-1-4939-9853-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2020
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Rapid Antigen and Antibody-Like Molecule Discovery by Staphylococcal Surface Display,nd screening after expression of the antibodies in a suitable organism and format. Here, a staphylococcal nanobody display is described that omits many the abovementioned intermediate steps and allows for simultaneous screening of multiple targets without prior knowledge nor expression of the binder
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Restriction-Free Construction of a Phage-Presented Very Short Macrocyclic Peptide Library,s containing very short sequences, however, poses certain problems for conventional restriction-based cloning procedures, which are rooted in the necessity to purify restricted library oligos. Herein, we present an alternative cloning method especially suitable for such very short sequences of about
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In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties,s can bind antigens independently and are the smallest reported adaptive immune recognition entities in the vertebrate kingdom. Sharing limited sequence homology with human immunoglobulin domains, their development and use as biotherapeutic agents require that they be humanized to minimize their pot
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Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens,says, and for therapeutics. Up to now (autumn 2018), eleven FDA/EMA-approved therapeutic antibodies were developed using phage display, including the world best-selling antibody adalimumab..A key to generate successfully human antibodies in vitro is the choice of the most appropriate antibody select
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