Titlebook: Genomics, Proteomics, and Clinical Bacteriology; Methods and Reviews Neil Woodford,Alan P. Johnson Book 2004 Humana Press 2004

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Genome Sequencing and Annotationted using clues derived from typical gene features, such as codon usage, ribosomal binding sequences, and bacterial initiation codons. Function of genes is predicted by homology searches performed against either public or well-established protein databases. This chapter discusses each of these stages in a genome-sequencing project.

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Real-Time PCRin real time is highly accurate compared with size analysis on gels. Analysis of the progress of the reaction allows accurate quantification of the target sequence over a very wide dynamic range, provided suitable standards are available. Finally, probe melting analysis can detect sequence variants including single base mutations.

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1064-3745 century, one does not have to be Nostradamus to predict that the current genomics and proteomics "revolution" will have an immense impact on medical bacteriology. This impact is already being re- ized in many academic departments, and although encroachment on routine diagnostic bacteriology, partic

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Exploring and Exploiting Bacterial Proteomeshe researcher with a tool to begin characterizing the functions of the vast proportion of “hypothetical” or “unknown” proteins elucidated from genome sequencing and database comparisons. For example, study of protein-protein, protein-ligand, protein-substrate, and protein-nucleic acid interactions f

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