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Titlebook: Genomics Protocols; Michael P. Starkey,Ramnath Elaswarapu Book 20011st edition Springer Science+Business Media New York 2001

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Springer Science+Business Media New York 2001
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Genomics Protocols978-1-59259-235-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
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https://doi.org/10.1007/978-3-642-73492-2Fluorescence . hybridization (FISH) provides a rapid means of placing labeled DNA segments into a wider genomic context. Mapping to banded metaphase chromosomes anchors clones for specific genes or markers in a well-recognized framework and provides a useful confirmation that clones belong to the expected region of interest.
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https://doi.org/10.1007/978-3-642-56883-1 F factor allows strict copy number control of the clones so that they are stably maintained at one to two copies per cell (.). The stability of the cloned DNA during propagation in . host is substantially higher in lower copy number vectors than in multi-copy counterparts.
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