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Titlebook: Genomics Protocols; Michael P. Starkey,Ramnath Elaswarapu Book 20011st edition Springer Science+Business Media New York 2001

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书目名称Genomics Protocols
编辑Michael P. Starkey,Ramnath Elaswarapu
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: Genomics Protocols;  Michael P. Starkey,Ramnath Elaswarapu Book 20011st edition Springer Science+Business Media New York 2001
描述We must unashamedly admit that a large part of the motivation for editing Genomics Protocols was selfish. The possibility of assembling in a single volume a unique and comprehensive collection of complete protocols, relevant to our work and the work of our colleagues, was too good an opportunity to miss. We are pleased to report, however, that the outcome is something of use not only to those who are experienced practitioners in the genomics field, but is also valuable to the larger community of researchers who have recognized the potential of genomics research and may themselves be beginning to explore the technologies involved. Some of the techniques described in Genomics Protocols are clearly not restricted to the genomics field; indeed, a prerequisite for many procedures in this discipline is that they require an extremely high throughput, beyond the scope of the average investigator. However, what we have endeavored here to achieve is both to compile a collection of procedures concerned with geno- scale investigations and to incorporate the key components of “bottom-up” and “top-down” approaches to gene finding. The technologies described extend from those traditionally recogn
出版日期Book 20011st edition
版次1
doihttps://doi.org/10.1385/159259235X
isbn_ebook978-1-59259-235-7Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2001
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Sequence-Based Detection of Single Nucleotide Polymorphisms,resence in both coding and noncoding regions, single nucleotide substitutions are probably the underlying cause of most phenotypic differences among humans. Therefore, the identification of SNPs in human genes will play an increasingly important role in analyzing genotype-phenotype correlations with
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Detection of Chromosomal Abnormalities by Comparative Genomic Hybridization,tasis. In addition, CGH can be used to compare different histologic components within one tumor, enabling a better understanding of the relation between phenotype and genotype, or to compare derivative cell lines with the original cell line.
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Construction of Transcript Maps by Somatic Cell/Radiation Hybrid Mapping,ms et al. (.) reported the generation of 174,172 human partial cDNA sequences and introduced the term . (ESTs). Since then, other major sequencing efforts (.) have brought the number of human ESTs in databases (.) to more than 1,400,000.
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Die Allgemeinbehandlung der Tuberkulose,resence in both coding and noncoding regions, single nucleotide substitutions are probably the underlying cause of most phenotypic differences among humans. Therefore, the identification of SNPs in human genes will play an increasingly important role in analyzing genotype-phenotype correlations with
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Diagnostik und Therapie der Pulpakrankheitens from the same individual) are removed. The . mismatch repair enzyme selection facilitates the removal of most of the mismatch-containing heterohybrids (.,.), therefore, DNA fragments from all IBD regions are isolated on the basis of their ability to form extended mismatch-free heterohybrids (doubl
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