Titlebook: Genome Editing; Current Technology A Shabir Hussain Wani,Goetz Hensel Book 2022 The Editor(s) (if applicable) and The Author(s), under excl

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Ezra Dessers,Mieke Van Gramberen time. Different Cas proteins have other binding prerequisites associated with the mechanistic interaction between Cas proteins and genomic DNA. The gene-specific protospacer-associated motif (PAM) for the common SpCas9 is NGG. Although this is a common motif frequently found in plant genic sequence

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Evaluating and Teaching Task Design,f crop species. The sole requirements for CRISPR/Cas-based gene editing are a guide RNA and a Cas protein. As a result, DNA-free methods employing Cas9/gRNA ribonucleoprotein (RNP) delivery have been developed to genome edit a variety of crop species and tissue types. In plants, common RNP-mediated

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