| 书目名称 | Genome Editing | | 副标题 | Current Technology A | | 编辑 | Shabir Hussain Wani,Goetz Hensel | | 视频video | | | 概述 | Guide to designing and implementing the CRISPR/Cas9 system for different plant systems.Practical resources for expert scientists and young researchers interested in CRISPR/Cas9 experiments.Chapters on | | 图书封面 |  | | 描述 | Over the last few decades, various techniques have been developed to alter the properties of plants and animals. While the targeted transfer of recombinant DNA into crop plants remains a valuable tool to achieve a desirable breeding outcome, integration of transgenes into the host genome has been random, which in part, leads to reduced acceptance of GMOs by the general population in some parts of the world. Likewise, methods of induced mutagenesis, such as TILLING, have the disadvantage that many mutations are induced per plant, which has to be removed again by expensive backcrossing. Advances in genome sequencing have provided more and more information on differences between susceptible and resistant varieties, which can now be directly targeted and modified using CRISPR/Cas9 technology. By selecting specific gRNAs occurrence of off-target modifications are comparatively low. ZFNs and TALENs- based approaches required re-engineering a new set of assembled polypeptides for every new target site for each experiment. The difficulty in cloning and protein engineering prevented these tools from being broadly adopted by the scientific community. Compared to these technologies, designi | | 出版日期 | Book 2022 | | 关键词 | Transgenic breeding; Genomic selection; CRISPR/Cas9; Cas endonucleases; gene targeting; biotic stress; abi | | 版次 | 1 | | doi | https://doi.org/10.1007/978-3-031-08072-2 | | isbn_softcover | 978-3-031-08074-6 | | isbn_ebook | 978-3-031-08072-2 | | copyright | The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerl |
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发表于 2025-3-21 17:20:45
