Titlebook: Genetically Incorporated Non-Canonical Amino Acids; Methods and Protocol Yu-Hsuan Tsai,Simon J. Elsässer Book 2023 The Editor(s) (if applic

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https://doi.org/10.1007/978-1-4842-9277-8ion. Here, we demonstrate this method by using cAMP-dependent protein kinase catalytic subunit alpha (PKA-Cα) as the model enzyme. The method should be applicable to other kinases, enabling their rapid and selective inhibition.

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Generation of Amber Suppression Cell Lines Using CRISPR-Cas9eaks (DSBs) and nonhomologous end joining (NHEJ) repair to target the PylT/RS expression cassette to the . safe harbor locus in human cells. .PylRS expression from this single locus is sufficient for efficient amber suppression when the cells are subsequently transfected transiently with a PylT/gene

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Fault-Tolerant System Technology,e describe a procedure for engineering photoactive antibody fragments that bind to their target antigen only after irradiation with 365 nm light. The procedure starts with identification of tyrosine residues in antibody fragments that are important for antibody–antigen binding and thus targets for r

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Dependability in Medicine and Neurologyalysts. Genetically encoded photo-redox cofactors endow photoenzymes with enhanced or novel activities that catalyze numerous transformations with high efficiency. Herein, we describe a protocol of repurposing photosensitizer proteins (.) through genetic code expansion to facilitate multiple photoca

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https://doi.org/10.1007/b102376oncanonical amino acids (ncAAs) by orthogonal aminoacyl-tRNA synthetase (aaRS)/tRNA pairs using nonsense codons, which could significantly expand new functionalities of proteins in both scientific and biomedical applications. Here, by hijacking the cysteine biosynthetic enzymes, we describe a method