Titlebook: Generation of cDNA Libraries; Methods and Protocol Shao-Yao Ying Book 2003 Humana Press 2003

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Construction of Size-Fractionated cDNA Library Assisted by an In Vitro Recombination Reaction,mprehensive analysis is most frequently carried out on a single-clone-for-single-gene basis, whereas gene discovery is done through the isolation of multiple cDNA clones for a single gene. Thus, current requirements for high-quality cDNA libraries suitable for comprehensive cDNA analysis are (1) hig

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Amplification of Representative cDNA Pools from Microscopic Amounts of Animal Tissue,demonstrated in our own experiments on differential display (.) and elsewhere in application of amplified cDNA as a probe for gene profiling by array technology (.–.). According to our experience in gene hunting in various biological models, amplified cDNA can substitute for normal, nonamplified cDN

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,mRNA/cDNA Library Construction Using RNA—Polymerase Cycling Reaction,hods. A minimum of several thousand cells is needed for an acceptable quality of RNA extraction. Because of tissue heterogeneity, these methods usually provided neither reliable nor reproducible results. Unfortunately, it is impossible to collect adequate amounts of pure or homogeneous samples for t

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,Screening Poly [dA/dT(−)] cDNA for Gene Identification,ion/subtraction, poly(dA)–poly(dT) hybrids will form randomly between unrelated cDNA templates. The removal of these hybrids causes the loss of cDNA templates. This phenomenon affects especially the low-copy cDNA templates, which represent most of the genes. To overcome this problem, we developed th

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