Titlebook: Generation of cDNA Libraries; Methods and Protocol Shao-Yao Ying Book 2003 Humana Press 2003

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https://doi.org/10.1057/9780230378827ailable. In essence, an adaptor with a defined sequence is attached to one end of the cDNA; then, the region between the adaptor and the known sequences is amplified by polymerase chain reaction (PCR). Since the initial publication in 1988 (.), RACE has greatly facilitated the cloning of new genes.

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Conversion and Environmental Conflictells. Some of them are expressed in most of the cells, but others are cell-or tissue-specific. It has been estimated that about 10,000 genes are expressed in a cell, but the abundance of their expression varies from 1 copy to 200,000 copies per cell (.). On average, the 10 most prevalent genes encod

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Bernhard Brouqueyre,Jean Michel Tauziaolation, characterization, and analysis of both eukaryotic and prokaryotic genes. However, the conventional methods of cDNA cloning require much effort to generate a cDNA library and then screen for a large number of recombinant phages or plasmid clones. There are three major limitations in these me

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https://doi.org/10.1057/9780230617858uman genomic sequence data. A number of attempts, which can be comprehensively termed “functional genomics,” are being carried out to decipher which parts of the human genome are transcribed, how the transcripts are spliced and translated, and what functions the eventual protein products conduct. Fo