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Titlebook: Gene Therapy of Cancer; Methods and Protocol Wolfgang Walther,Ulrike Stein Book 20001st edition The Editor(s) (if applicable) and The Autho

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书目名称Gene Therapy of Cancer
副标题Methods and Protocol
编辑Wolfgang Walther,Ulrike Stein
视频video
概述Includes supplementary material:
丛书名称Methods in Molecular Medicine
图书封面Titlebook: Gene Therapy of Cancer; Methods and Protocol Wolfgang Walther,Ulrike Stein Book 20001st edition The Editor(s) (if applicable) and The Autho
描述Since the discovery of the molecular structure of genes and the unveiling of the molecular basis of numerous human diseases, scientists have been fas- nated with the possibility of treating certain diseases by transducing foreign DNA into the affected cells. Initially, it was proposed that the foreign DNA could either replace defective nonfunctional genes, or code for therapeutic proteins. This concept has evolved into the rapidly growing field of gene therapy. Even though surgery, radiotherapy, and chemotherapy are widely ava- able and routinely used for cancer treatment, these therapies fail to cure approximately 50 percent of cancer patients. Therefore, since it is a disease characterized by aberrant gene expression, cancer has been a target of gene therapy research since the inception of this treatment modality. Numerous cancer gene therapy strategies are currently being investigated, including gene replacement therapy, the regulation of gene expression to modulate immu- logical responses to tumors, the direct killing of tumor cells, and direct int- ference with tumor growth. In this context, gene transfer systems, tumor-specific expression vectors, and novel therapeutic genes
出版日期Book 20001st edition
版次1
doihttps://doi.org/10.1385/1592590861
isbn_softcover978-0-89603-843-1
isbn_ebook978-1-59259-086-5Series ISSN 1543-1894 Series E-ISSN 1940-6037
issn_series 1543-1894
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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Immunizing Potential of Cytokine-Transduced Tumor Cellsermined by the type of cytokine, its quantity and activity, the histotype of the tumor and the molecules it releases, and its extracellular matrix (.). However, the relevant point is that a cascade of events other than tumor debulking are initiated by the transduced cytokines. Infiltration of differ
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Particle-Mediated Gene Transfer into Dendritic Cells consisting of antibody responses specific for conformational determinants, as well as, antigen-specific CD8.cytotoxic T cells and CD4.T-helper cells. For this reason, it represents an attractive novel approach for the clinical development of prophylactic and therapeutic vaccines against certain inf
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7 In Vitro Methods for Evaluation of ,-Based Anticancer Gene Therapyudies; 3) a large throughput of P450 genes and drugs can readily be tested to identify novel prodrug activation gene and prodrug combinations. This chapter describes growth inhibition and cytotoxicity assays that can be used to rapidly characterize the drug sensitivity of tumor cell lines transduced
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Combined Adenoviral Transfer of Tumor Suppressor and Cell-Cycle Genes for Tumor-Cell Apoptosis choice, which gene combination will be particularly efficient, depends on the pattern of mutated genes. We have recently reported that the cotransfer of . and . leads to a better induction of apoptosis and reduction of tumor growth than the transfer of either gene alone (.). In addition, it seems t
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Inhibition of Cell Growth by Antisense Oligonucleotides Targeting the Growth-Related Protein Kinase nduce apoptosis of cancer cells. Although these various approaches have not been validated clinically, these strategies are likely to identify compounds with less side effects compared to standard chemotherapeutic agents.
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Climate Science and Paleoclimatology, choice, which gene combination will be particularly efficient, depends on the pattern of mutated genes. We have recently reported that the cotransfer of . and . leads to a better induction of apoptosis and reduction of tumor growth than the transfer of either gene alone (.). In addition, it seems t
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