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Titlebook: Gene Therapy Protocols; Paul D. Robbins Book 19971st edition Springer Science+Business Media New York 1997

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Methods for Retrovirus-Mediated Gene Transfer into Primary T-Lymphocytes,ate for the transduction of primary lymphocytes, because gene transfer is mediated by nonimmunogenic vectors and stable vector integration in the target genome is achieved. Stable integration in cells undergoing clonal expansion ensures that the foreign genetic material will be faithfully transmitted to the cells’ progeny.
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https://doi.org/10.1007/978-3-658-26645-5us types of cells/tissues have been successfully genetically modified and are potential targets for retroviral mediated gene therapy (.).The successful use of these tissues in gene therapy relies on methods to cultivate and transplant the cells after genetic modification.
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Methods for the Construction of Retroviral Vectors and the Generation of High-Titer Producers,re when the foreign genetic material must be faithfully transmitted to the progeny of the transduced parental cell, murine leukemia virus (MLV)-based vectors accommodate numerous modifications, thus providing a plastic tool that can be tailored for very diverse applications.
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Methods for the Use of Retroviral Vectors for Transfer of the CFTR Gene to Airway Epithelium,oride secretion lead to dehydration of the fluids on the airway surface and, in turn, this leads to chronic infections and severe damage. The severity of CF lung disease and the potential accessibility of the airways to gene transfer vectors has led to proposals that gene therapy be applied for the treatment of CF lung disease (.).
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Methods for the Use of Genetically Modified Keratinocytes in Gene Therapy,us types of cells/tissues have been successfully genetically modified and are potential targets for retroviral mediated gene therapy (.).The successful use of these tissues in gene therapy relies on methods to cultivate and transplant the cells after genetic modification.
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John P. Houghton,Andrew Stevensthe generation of recombinant AAV vectors, it is of value to briefly discuss the structure and life cycle of this unique virus. Detailed and more extensive reviews that describe the biology of adeno-associated virus are also available (.-.).
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