Titlebook: Gene Knockout Protocols; Martin J. Tymms,Ismail Kola Book 2001 Humana Press 2001

大范围流行

充满人

Choice of Mouse Strains for Gene Targeting,train of mice for mating contribute to the genetic composition of the targeted mouse, whereas the strain of host embryos is only relevant in that it must be compatible with the choice of ES cells, otherwise the ability of the chimeric mice to provide germline transmission of the ES cell genome may be compromised.

Conscientious

Intact

of development. This system can also be used to facilitate transgene activation/inactivation in vivo, deletions of large stretches of genomic DNA, chromosomal translocation and subtle alterations to genes and/or their regulatory sequences in vivo.

缓解

带来墨水

Mobile

Classification of Multiword Expressions,). Gain-of-function mutations are generally generated by cDNA overexpression and in this chapter we review the status of heterologous gene expression systems that can be used for this purpose in ES cells.

疏远天际

天真

GENUS