Titlebook: Gene Expression Analysis; Methods and Protocol Nalini Raghavachari,Natàlia Garcia-Reyero Book 2018 Springer Science+Business Media, LLC, pa

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Juan Albarracin-Jordan,Thérèse Bouchardssues in an effort to unravel biological pathways associated with development and disease. The advent of technologies is now enabling the study of such comprehensive transcriptional characterization of mRNA, miRNA, lncRNA, and small RNA in a robust and successful manner. Transcriptomic strategies ar

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Children‘s Rights in International Politicsore the transcriptome and to better understand biological systems and their perturbation. In this chapter we provide an overview of microarray and massively parallel sequencing technologies and their application to gene expression analysis. We discuss factors that impact expression data generation a

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Deborah L. Best,Judith L. Gibbonsst frequently performed experimental techniques in molecular biology. The development of the RNA-Sequencing (RNA-Seq) method allows an unprecedented opportunity to analyze expression of protein-coding, noncoding RNA and also de novo transcript assembly of a new species or organism. However, the plan

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Dialogue and Collaboration: Girls and Boys,pplications can benefit from reducing the number of processing steps including eliminating the poly(A) selection and ribosomal depletion steps. When performing targeted capture, we have found that we can eliminate the upfront poly(A) selection/ribosomal depletion steps that cause bias in standard mR

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https://doi.org/10.1007/978-94-011-8902-6haracterize the composition and structure of microbial communities. Preparing genomic DNA for sequencing of such gene fragments on Illumina sequencers can be performed in a straightforward, two-stage PCR method, described herein. The protocol described allows for up to 384 samples to be sequenced si