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Titlebook: Gene Expression Analysis; Methods and Protocol Nalini Raghavachari,Natàlia Garcia-Reyero Book 2018 Springer Science+Business Media, LLC, pa

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书目名称Gene Expression Analysis
副标题Methods and Protocol
编辑Nalini Raghavachari,Natàlia Garcia-Reyero
视频video
概述Includes cutting-edge methods an protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Gene Expression Analysis; Methods and Protocol Nalini Raghavachari,Natàlia Garcia-Reyero Book 2018 Springer Science+Business Media, LLC, pa
描述.This volume provides experimental and bioinformatics approaches related to different aspects of gene expression analysis. Divided in three sections chapters detail wet-lab protocols, bioinformatics approaches, single-cell gene expression, highly multiplexed amplicon sequencing, multi-omics techniques, and targeted sequencing. Written in the highly successful .Methods in Molecular Biology .series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.. Authoritative and cutting-edge, .Gene Expression Analysis: Methods and Protocols .aims provide useful information to researchers worldwide..
出版日期Book 2018
关键词exportin-5; nucleocytoplasmatic transport factors; chromatin modiification; histone acetylation; cytosin
版次1
doihttps://doi.org/10.1007/978-1-4939-7834-2
isbn_softcover978-1-4939-9299-7
isbn_ebook978-1-4939-7834-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2018
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Dialogue and Collaboration: Girls and Boys, matched samples including fresh frozen (FF) or formalin-fixed, paraffin-embedded (FFPE) from tumor/normal tissues we generated high-quality data using a protocol that does not require upfront ribosomal depletion or poly(A) selection. Using SureSelect. RNA Direct protocol (RNA Direct) workflow, we f
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https://doi.org/10.1007/978-3-663-02375-3periment, which often leads to impreciseness for example, target mRNA transcribed from the same source should be identical every time. In such cases, developing an optimized protocol for microarray platform to study the expression profiling of differentially regulated genes is a challenging task. Th
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fidence interactions were validated in literature, and our inferred network captured two major cell death pathways by identifying BCL2L11 and PMAIP1 as key interacting players for the intrinsic apoptosis pathway, and TAXBP1 and TNFAIP3 for the extrinsic apoptosis pathway. Our inferred apoptosis netw
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A Guide for Designing and Analyzing RNA-Seq Data,xperiments. We describe critical aspects of wet lab experiments such as RNA isolation, library preparation and the initial design of an experiment. Further, we provide a step-by-step description of the bioinformatics workflow for different steps involved in RNA-Seq data analysis. This includes power
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SureSelectXT RNA Direct: A Technique for Expression Analysis Through Sequencing of Target-Enriched matched samples including fresh frozen (FF) or formalin-fixed, paraffin-embedded (FFPE) from tumor/normal tissues we generated high-quality data using a protocol that does not require upfront ribosomal depletion or poly(A) selection. Using SureSelect. RNA Direct protocol (RNA Direct) workflow, we f
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