Titlebook: Gene Cloning; The Mechanics of DNA David M. Glover Book 1984 D. M. Glover 1984 DNA.bacteria.biology.cells.cloning.gene.genes.molecule.mutag

使乳化

Expression of cloned DNAs in , plasmids,al and translational machinery of the host cell. The bacterium . is a natural choice for such studies because of our detailed knowledge of the molecular biology of its gene expression. A vast amount of effort has now been put into the development of plasmid vectors to maximize the expression of fore

自恋

The physical characterization of cloned DNA segments and their counterparts within chromosomes,ouse, for example, contains approximately 3 × 10. kb of DNA. The purification and characterization of a gene such as that encoding .-globin was impossible before the advent of such techniques. The techniques for gene cloning not only enable a gene to be purified but also permit the amplification of

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一夫一妻制

Foolproof

Transport and Transformation Processesc acid hybridization or enabling the production of large amounts of particular gene products. Research on the bacterial plasmids has had a similar history. The discovery and rationalization of the mechanism whereby F factors promote bacterial conjugation was central to the development of . genetics.

Dysplasia

Virtual screening by molecular dockingM and .R. A polypeptide which governs the specificity of the system is determined by .S. The gene product of .M is the modification enzyme which also interacts with the product of the .R gene, the restriction endonuclease, in the cleavage process. In the above examples the phage grown on strain K wo

含铁

https://doi.org/10.1007/1-4020-5001-1as a result of laboratory experimentation. The fear was that a potentially dangerous gene could be accidentally transferred to a variety of bacteria if cloned upon a vector capable of conjugation. The conjugative plasmids are capable, not only of their own transmission from one cell to another, but

irreducible

Rupert P. M. Negus,Frances R. Balkwillir structural genes, and why gene transcripts in the nuclei of most higher organisms are very much larger and more heterogeneous than the functional mRNAs in the cell cytoplasm. Our picture of gene control in eukaryotes is, however, still very incomplete in spite of the information explosion generat

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