Titlebook: Gene Cloning; The Mechanics of DNA David M. Glover Book 1984 D. M. Glover 1984 DNA.bacteria.biology.cells.cloning.gene.genes.molecule.mutag

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Stefano Moro,Magdalena Bacilieribout half are essential. The nonessential genes can be replaced with foreign DNA and the recombinant genome DNA can then be propagated in .. This chapter will trace the development of a versatile series of vectors for different cloning problems.

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Gene cloning in fungi and plants, cell wall. The spheroplasts, so produced, can be maintained in isotonic solutions of sorbitol and DNA uptake can be promoted by the addition of polyethylene glycol. The cell wall regenerates if the treated spheroplasts are plated in 3% agar.

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Tracy Fischer-Smith,Jay Rappaportign DNA in . It is outside the scope of this chapter to fully document these efforts, which have been reviewed elsewhere [1], Instead we will look at essential features of such heterologous expression systems.

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Expression of cloned DNAs in , plasmids,ign DNA in . It is outside the scope of this chapter to fully document these efforts, which have been reviewed elsewhere [1], Instead we will look at essential features of such heterologous expression systems.

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The principles of cloning DNA,ial plasmids. Of the bacteriophage themselves, the . phage . is perhaps the best characterized. The interaction of phage . with the host cell is a particularly fruitful area of study, as here are a set of genes which can either direct cell lysis or become stably associated with the host chromosome i

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