Titlebook: Gastrointestinal Physiology and Diseases; Methods and Protocol Andrei I. Ivanov Book 2016 Springer Science+Business Media New York 2016 gas

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Organotypical Tissue Cultures from Fetal and Neonatal Murine Colonufficiently using cell lines or two-dimensional cell cultures..Here, we describe an easy to produce three-dimensional organotypical explants culture from fetal and neonatal murine colon. This model is suitable for in vitro testing of intestinal function or the evaluation of developmental or pathological processes.

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Physics and Chemistry of Atmospheric Ions,on is used for the analysis of adenine nucleotide metabolites, whereas electrochemical detection is used for the analysis of multiple amino acid metabolites. These methodologies provide platforms for further characterization of the metabolic changes that occur during gastrointestinal inflammation.

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HPLC-Based Metabolomic Analysis of Normal and Inflamed Guton is used for the analysis of adenine nucleotide metabolites, whereas electrochemical detection is used for the analysis of multiple amino acid metabolites. These methodologies provide platforms for further characterization of the metabolic changes that occur during gastrointestinal inflammation.

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https://doi.org/10.1007/3-540-37698-4ct expression patterns of mature fundic cell types seen in vivo, thus allowing for long-term in vitro studies of gastric epithelial cell physiology, regeneration, and bacterial-host interactions. Here, we describe the development and culture of mFGOs, co-cultured with ISMCs.