Titlebook: Gastrointestinal Physiology and Diseases; Methods and Protocol Andrei I. Ivanov Book 2016 Springer Science+Business Media New York 2016 gas

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https://doi.org/10.1007/978-3-642-82289-6e HIF (hypoxia inducible factor) transcription factor, which regulates adaptive pathways including genes controlling glycolytic metabolism and angiogenesis. In adequately oxygenated tissues (i.e. normoxia), the HIF protein is constantly produced, but oxygen-dependent enzymes called prolyl-hydroxylas

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Coleman J. Major,Richard W. Tockcells are maintained by self-renewal, while giving rise to all major cell types of the intestinal epithelium. This protocol allows the study of gene function in intestinal epithelial homeostasis and pathophysiology and can be extended to epithelial organoids derived from other internal mouse and human organs.

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https://doi.org/10.1007/978-94-009-0923-6flies have been developed. We have developed techniques to generate germ-free ., monoassociate them with candidate bacteria, and assess ensuing physiological responses within the gut tissue that include the generation of reactive oxygen species and cell proliferation.

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Detecting Reactive Oxygen Species Generation and Stem Cell Proliferation in the Drosophila Intestineflies have been developed. We have developed techniques to generate germ-free ., monoassociate them with candidate bacteria, and assess ensuing physiological responses within the gut tissue that include the generation of reactive oxygen species and cell proliferation.

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Near-Infrared Fluorescence Endoscopy to Detect Dysplastic Lesions in the Mouse Colonesions developed in murine colitis. Our studies suggest that NIRF endoscopy is a specific and sensitive technique that provides a unique opportunity to analyze early stages of tumorigenesis in animal models of colon cancer and to perform surveillance colonoscopy in patients with colitis-associated colon cancer.

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Book 2016ogical functions of the gastrointestinal system and to model the most common digestive diseases. The chapters focus on diverse research topicsincluding ex vivo systems to study gastrointestinal development and functions,in vivo imaging of the gastrointestinal tract, isolation and characterizationof

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Co-culture of Gastric Organoids and Immortalized Stomach Mesenchymal Cellsct expression patterns of mature fundic cell types seen in vivo, thus allowing for long-term in vitro studies of gastric epithelial cell physiology, regeneration, and bacterial-host interactions. Here, we describe the development and culture of mFGOs, co-cultured with ISMCs.