Titlebook: G Protein-Coupled Receptor Signaling; Methods and Protocol Mario Tiberi Book 2019 Springer Science+Business Media, LLC, part of Springer Na

暖昧关系

薄荷醇

键琴

失眠症

ABOUT

Measuring GPCR Stoichiometry Using Types-1, -2, and -3 Bioluminescence Resonance Energy Transfer-Bas interactions from non-specific associations that inevitably result from constraining proteins in cellular membranes. In our experience, concordant data obtained in two or more of these assays, benchmarked with suitable controls, strongly predict receptor stoichiometry.

柱廊

Quantification and Comparison of Signals Generated by Different FRET-Based cAMP Reportersthe underlying cAMP change. In this chapter, we provide examples of the problems that may arise when using normalized FRET data and present a method based on the conversion of FRET ratio changes into actual cAMP concentrations that mitigates these issues.

heckle

山羊

Transradial Approach for Strokeies do not discriminate ligand binding on GPCR monomers, homo- or hetero-oligomers. To address this issue, we recently developed a new assay based on a time-resolved FRET method that is easy to implement and that can focus on ligand binding specifically on the hetero-oligomer.

FLAT

Hippocampus

Molecular Dynamic Simulations to Probe Water Permeation Pathways of GPCRsthe MD simulations of the rhodopsin [preparation of initial systems, condition files for MD simulation package GROMACS, and data analysis]. The data analysis includes the root mean square deviation (RMSD) and mapping of accessibility of water molecules.