Titlebook: G Protein-Coupled Receptor Screening Assays; Methods and Protocol Duarte Miguel F. Prazeres,Sofia Aires M. Martins Book 2015 Springer Scien

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Quantifying GPCR Internalization: A Focus on the Kisspeptin Receptorhe use of immunofluorescence and imaging techniques as well as flow cytometry. The techniques described use the FLAG-tagged kisspeptin receptor (KISS1R) as an example but are equally applicable to any other GPCR.

Provenance

喃喃诉苦

Seizure

Measurement of Surface-Mediated Ca2+ Transients on the Single-Cell Level in a Microfluidic Lab-on-a-er cells or particles, and the simultaneous analysis of the induced cytosolic calcium signals. The method is based on a microfluidic lab-on-a-chip environment that allows for contactless cell and particle handling by dielectrophoretic forces.

增强

https://doi.org/10.1007/978-3-0348-6378-0luorescence plate reader platforms. For such assays a considerable amount of cells expressing the desired biosensor is needed. A method to achieve sufficient and reproducible level of cAMP biosensor protein expression with the means of BacMam transduction system is the subject of this chapter.

modest

cAMP Assay for GPCR Ligand Characterization: Application of BacMam Expression Systemluorescence plate reader platforms. For such assays a considerable amount of cells expressing the desired biosensor is needed. A method to achieve sufficient and reproducible level of cAMP biosensor protein expression with the means of BacMam transduction system is the subject of this chapter.

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crescendo

独特性

https://doi.org/10.1007/978-3-658-24813-0 activity integrates this complexity. In this assay protocol, we describe how the xCELLigence RTCA HT impedance-based platform which can be used for functional cell-based GPCR assays can be utilized for GPCR screening.

Minutes

https://doi.org/10.1007/978-3-658-01505-3gand-directed desensitization of the receptor. DMR antagonist reverse assays manifest biased antagonism. DMR profiling using distinct probe-modulated cells detects the biased agonism in the context of self-referenced pharmacological activity map.