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Titlebook: G Protein-Coupled Receptor Screening Assays; Methods and Protocol Duarte Miguel F. Prazeres,Sofia Aires M. Martins Book 2015 Springer Scien

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书目名称G Protein-Coupled Receptor Screening Assays
副标题Methods and Protocol
编辑Duarte Miguel F. Prazeres,Sofia Aires M. Martins
视频video
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice.Includes supplementary material:
丛书名称Methods in Molecular Biology
图书封面Titlebook: G Protein-Coupled Receptor Screening Assays; Methods and Protocol Duarte Miguel F. Prazeres,Sofia Aires M. Martins Book 2015 Springer Scien
描述. This volume. .explores the considerable efforts that have been directed towards the development of G Protein-Coupled Receptors (GPCR) screening assays in order to disclose GPCR acting compounds, elucidate signaling mechanisms or evaluate compound’s efficacy. New discoveries in the field, along with the widely recognized need for better and safer pharmaceutical drugs constitute the main motivation for this book. Readers, both beginners and experienced researchers, will receive an updated overview of not only the established, but also the innovative technologies that promise to advance GPCR drug research. This book is organized into two major parts: the introductory part discusses the necessary foundations for the understanding of GPCR action and the rationale behind the design of the available screening assays; and part two provides detailed protocols for different screening approaches. Written in the highly successful .Methods in Molecular Biology .series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory..Practical and innovative, .G Protein-Coupled Receptor Screening Assays: Metho
出版日期Book 2015
关键词G-Protein Coupled Receptors; GPCR signaling; GPCR-active drugs; drug research; high content screening; hi
版次1
doihttps://doi.org/10.1007/978-1-4939-2336-6
isbn_softcover978-1-4939-5580-0
isbn_ebook978-1-4939-2336-6Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media New York 2015
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https://doi.org/10.1007/978-3-0348-6378-0d to estimate the biological activity of various GPCR-specific agents. Förster resonance energy transfer (FRET) biosensors have been around for almost 10 years and became increasingly popular for cAMP detection. Ratiometric sensitized emission assay of a FRET biosensor can easily be implemented on f
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Margret Liehn,Hannelore Schlautmannause their cognate G protein signaling pathways are often unknown. In this methodological chapter, we describe the use of constitutive activity, that is, the inherent ability of receptors to couple to their cognate G proteins in the absence of ligand, to inform the development of high-throughput scr
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