Titlebook: G Protein-Coupled Receptor Genetics; Research and Methods Craig W. Stevens Book 2014 Springer Science+Business Media New York 2014 G Protei

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Investigation of G Protein-Coupled Receptor Function and Regulation Using Antisensecting proteins, or modulate downstream signaling molecules. Where appropriate agents are unobtainable, alternative molecular methods have been developed to determine the specific roles that individual proteins play in GPCR regulation and signaling. One such method utilizes RNA interference (RNAi) to

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Viral Infection for G Protein-Coupled Receptor Expression in Eukaryotic Cellspled receptors (GPCRs) or other proteins of interest. Adenoviruses are non-enveloped viruses containing a linear double-stranded DNA genome. Their life cycle does not normally involve integration into the host genome, rather they replicate as episomal elements in the nucleus of the host cell, and co

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Design of Super-arrestins for Gene Therapy of Diseases Associated with Excessive Signaling of G Proting also underlies certain acquired pathological conditions. The coupling of most GPCRs to their cognate G proteins is stopped by the natural desensitization mechanism, which includes receptor phosphorylation by GRKs followed by arrestin binding to active phosphoreceptor. Elucidation of the molecula