Titlebook: G Protein Signaling; Methods and Protocol Alan V. Smrcka Book 2004 Humana Press 2004

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1064-3745 f physiological processes and are consequently the targets of many pharmaceuticals. In G Protein Signaling: Methods and Protocols, leading researchers describe in detail key methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole anima

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https://doi.org/10.1007/978-94-6091-734-9histidine tag (H6) is incorporated into the expression vector and utilized for affinity purification on a Ni-NTA column. Subsequently, an anion exchange column is employed to further purify the protein.

GLUT

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De fysiologie van de glucosehuishouding, of the reporter luciferase under the control of a modified serum-responsive element (SRE) in a cell line derived from mice lacking Gα. that are transfected with a GPCR of interest, allows for easy, fast, and valid determination of the coupling of the GPCR to Gα..

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https://doi.org/10.1007/978-3-322-97190-6s through RGS domain of about 120 amino acids. Thus, the overexpression of the RGS domain in cells can specifically block the signalling pathways that are mediated by the interacting G proteins. The method is particularly useful to differentiate between G.-mediated and G.-mediated pathways.

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Assay for G Protein-Dependent Activation of Phospholipase C β Using Purified Protein Componentsivity PLC activity and its ability to be regulated by βγ and α. subunits. It can also be used to assess the functionality of the components after modification by mutagenesis, chemical modification, or in the presence of competing molecules.

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