| 书目名称 | G Protein Signaling | | 副标题 | Methods and Protocol | | 编辑 | Alan V. Smrcka | | 视频video | http://file.papertrans.cn/381/380002/380002.mp4 | | 概述 | Includes supplementary material: | | 丛书名称 | Methods in Molecular Biology | | 图书封面 |  | | 描述 | G proteins and G protein-coupled receptors are ubiquitously expressed proteins that regulate a wide range of physiological processes and are consequently the targets of many pharmaceuticals. In G Protein Signaling: Methods and Protocols, leading researchers describe in detail key methods for investigating G protein signaling from a variety of perspectives ranging from in vitro biochemistry to whole animal studies. The authors focus on the mechanisms of G protein and G protein-coupled receptor function and the roles of G protein subunits in cell biology and disease. Among the readily reproducible techniques presented are those for the purification of G proteins and effector enzymes, assays of these purified G proteins and effector enzymes, and for the study of G protein interactions with effectors in intact cells. Additional methods are provided for assaying G protein-coupled receptor structure, function, and localization, for studying the physiological roles for endogenous G proteins, and for examining lipid and phosphate modifications of RGS proteins. Each fully tested protocol includes a background introduction explaining the principle behind the technique, equipment and reagent | | 出版日期 | Book 2004 | | 版次 | 1 | | doi | https://doi.org/10.1385/1592594301 | | isbn_softcover | 978-1-61737-381-7 | | isbn_ebook | 978-1-59259-430-6Series ISSN 1064-3745 Series E-ISSN 1940-6029 | | issn_series | 1064-3745 | | copyright | Humana Press 2004 |
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