Titlebook: Epizootic Hemorrhagic Disease Virus; Methods and Protocol Carrie Batten Book 2024 The Editor(s) (if applicable) and The Author(s), under ex

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Virus Neutralization Test for Detecting and Quantifying Serum-Neutralizing Antibodies to Epizootic sitive and specific test. As with most neutralization assays, the EHDV VNT does not react with all virus-targeting antibodies, but specifically with those antibodies that bind to VP2, the outermost capsid structural protein of the virus. The interaction between VP2 and neutralizing antibodies can bl

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Chronological

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Real-Time RT-PCR for the Diagnosis of Epizootic Hemorrhagic Disease Virus,seases such as epizootic hemorrhagic disease (EHD). Here we provide a protocol for the detection of epizootic hemorrhagic disease virus (EHDV) genetic material in blood and tissue samples, using a real-time RT-PCR that targets a conserved region in segment 9 of the EHDV genome. This protocol can be

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Real-Time RT-PCR Assays for Typing of Epizootic Hemorrhagic Disease Virus,d confirmation of disease. Several real-time RT-PCR methods have been reported over the last 10 years. In this chapter, we describe seven duplex real-time RT-PCR assays to amplify part of genome segment 2 of EHDV to enable serotype identification. The assay includes the detection of an endogenous co

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Rapid Laboratory Diagnosis of Epizootic Hemorrhagic Disease Virus Using a Loop-Mediated Isothermal f viral diseases of livestock. A major advantage of RT-LAMP is that it can be used either as a rapid field test or as a high-throughput screening tool in veterinary laboratories, with sensitivity comparable to the real-time RT-PCR assay. Unlike conventional or qPCR, RT-LAMP uses a strand displacemen

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End-Point RT-PCR Assays for Detection and Typing of Epizootic Hemorrhagic Disease Virus,h this virus. Viral genome testing can be performed on whole blood under anticoagulant, spleen, and bloody organ homogenates from ruminants. It can also be performed on cell culture following viral isolation tests. Various so-called classical or end-point RT-PCRs will be described, which permit the

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Whole Genome Sequencing of Epizootic Hemorrhagic Disease Virus, viral genomes. Whole genome sequencing of segmented viruses, such as epizootic hemorrhagic disease virus (EHDV), provides insights into the molecular epidemiology as well as such viral evolutionary mechanisms as genetic reassortment. Here, we present a detailed method for obtaining full genome sequ

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