Titlebook: Epizootic Hemorrhagic Disease Virus; Methods and Protocol Carrie Batten Book 2024 The Editor(s) (if applicable) and The Author(s), under ex

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https://doi.org/10.1007/978-3-658-31222-0sitive and specific test. As with most neutralization assays, the EHDV VNT does not react with all virus-targeting antibodies, but specifically with those antibodies that bind to VP2, the outermost capsid structural protein of the virus. The interaction between VP2 and neutralizing antibodies can bl

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https://doi.org/10.1007/978-3-642-57621-8ation lines in a semisolid matrix. Here we describe the preparation of agar gel plates, the method to test serum samples by AGID for the presence of EHDV antibodies, and the interpretation of test results. This test has known cross-reactivity to bluetongue antibodies; therefore positive samples by t

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https://doi.org/10.1007/978-3-662-67801-5d confirmation of disease. Several real-time RT-PCR methods have been reported over the last 10 years. In this chapter, we describe seven duplex real-time RT-PCR assays to amplify part of genome segment 2 of EHDV to enable serotype identification. The assay includes the detection of an endogenous co

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https://doi.org/10.1007/978-3-642-05064-0f viral diseases of livestock. A major advantage of RT-LAMP is that it can be used either as a rapid field test or as a high-throughput screening tool in veterinary laboratories, with sensitivity comparable to the real-time RT-PCR assay. Unlike conventional or qPCR, RT-LAMP uses a strand displacemen

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https://doi.org/10.1007/978-3-658-43004-7 viral genomes. Whole genome sequencing of segmented viruses, such as epizootic hemorrhagic disease virus (EHDV), provides insights into the molecular epidemiology as well as such viral evolutionary mechanisms as genetic reassortment. Here, we present a detailed method for obtaining full genome sequ

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https://doi.org/10.1007/978-3-211-77141-9ding of the viral infection and replication kinetics within these insects, including the proportion of the insect population that are able to support virus transmission. Here, we describe methods for the infection of . with EHDV in the laboratory via oral infection using an artificial membrane syste