Titlebook: Epizootic Hemorrhagic Disease Virus; Methods and Protocol Carrie Batten Book 2024 The Editor(s) (if applicable) and The Author(s), under ex

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https://doi.org/10.1007/978-3-531-90655-3quality of starting material. Here we describe the preparation and isolation of epizootic hemorrhagic disease virus (EHDV) from blood and tissue samples in tissue culture flasks (TCFs) through the inoculation of susceptible cell lines including Vero, BHK, and KC cells.

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https://doi.org/10.1007/978-3-662-67801-5d confirmation of disease. Several real-time RT-PCR methods have been reported over the last 10 years. In this chapter, we describe seven duplex real-time RT-PCR assays to amplify part of genome segment 2 of EHDV to enable serotype identification. The assay includes the detection of an endogenous control gene—beta-actin.

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Real-Time RT-PCR Assays for Typing of Epizootic Hemorrhagic Disease Virus,d confirmation of disease. Several real-time RT-PCR methods have been reported over the last 10 years. In this chapter, we describe seven duplex real-time RT-PCR assays to amplify part of genome segment 2 of EHDV to enable serotype identification. The assay includes the detection of an endogenous control gene—beta-actin.

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Managing Risk When Working with Orbiviruses with a Special Emphasis on Epizootic Hemorrhagic Diseassessment provides stepwise guidance for completing a risk assessment, from information gathering and identifying hazards to evaluating the risks, developing, and implementing controls and review..To support the development of a mature safety culture within laboratories, it is important that all staf

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Recognition of Field Signs, Necropsy Procedures, and Evaluation of Macroscopic Lesions of Cervids Iong ruminant hosts and . species vectors. A variety of domestic and wild ruminant species are susceptible to EHDV infection, but infection outcome is highly variable between species, as well as between individuals of the same species. Thus, this disease system inherently operates at the wildlife-liv

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Purification of Epizootic Hemorrhagic Disease Virus (and Other Orbiviruses) Particles from Infectedosts EHDV, like bluetongue virus (BTV), it has mainly been associated with infection of endothelial cells of capillaries as well as leukocyte subsets. Furthermore, EHDV infects and replicates within its biological vector, . biting midges and .derived cells. A wide range of common laboratory cell lin

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Epizootic Hemorrhagic Disease Virus Titration,ral component of epizootic hemorrhagic disease virus (EHDV) research, including estimating infectivity, calculating multiplicity of infection, and confirming virus propagation in cell culture. However, the ability to quantify infectious EHDV is also critical for disease control, particularly in the