Titlebook: Extracellular Vesicles in Diagnosis and Therapy; Maurizio Federico,Barbara Ridolfi Book 2022 The Editor(s) (if applicable) and The Author(

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https://doi.org/10.1007/978-3-319-11155-1, and are critically involved in the pathophysiology of various diseases, including tumors. In order to study the interaction of tumor cell-derived EVs with their target cells and to investigate their biological functions in comparison to other tumor cell-released factors, efficient isolation of EVs

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Isolation of Circulating Extracellular Vesicles by High-Performance Size-Exclusion Chromatographyifferent cell sources that constitute an attractive target for biomarker studies. However, there is no consensus on the best approach to isolate EVs from blood. Non-EV proteins and lipoproteins in plasma/serum tend to contaminate isolated EVs and confound functional experiments. Here we describe a s