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Titlebook: Extracellular Vesicles in Diagnosis and Therapy; Maurizio Federico,Barbara Ridolfi Book 2022 The Editor(s) (if applicable) and The Author(

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书目名称Extracellular Vesicles in Diagnosis and Therapy
编辑Maurizio Federico,Barbara Ridolfi
视频video
概述Includes cutting-edge techniques.Provides step-by-step detail essential for reproducible results.Contains key implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Extracellular Vesicles in Diagnosis and Therapy;  Maurizio Federico,Barbara Ridolfi Book 2022 The Editor(s) (if applicable) and The Author(
描述This detailed book provides an exhaustive picture of current methods to detect, isolate, and analyze extracellular vesicles (EVs) from diverse sources, now seen as potential disease biomarkers as well as tools for the development of new therapies. Beginning with a section on detection and isolation of EVs, the volume continues with chapters covering different methods to isolate and quantify EVs from specialized tissues/organs and body fluids, methods devoted to analyzing EV components, as well as cutting-edge methods to engineer EVs. Written for the highly successful .Methods in Molecular Biology. series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. .Authoritative and practical, .Extracellular Vesicles in Diagnosis and Therapy. serves as an ideal guide for researchers seeking to learn more about the complexities of cell-to-cell communication..
出版日期Book 2022
关键词Disease biomarkers; EV detection and isolation; Macromolecule delivery; Biofluids; Vesicle engineering; I
版次1
doihttps://doi.org/10.1007/978-1-0716-2341-1
isbn_softcover978-1-0716-2343-5
isbn_ebook978-1-0716-2341-1Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightThe Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Busines
The information of publication is updating

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https://doi.org/10.1007/978-3-642-66092-4n. TEM enables visualization of the exosomes which often present a cup-shaped morphology. Western blot is used to detect commonly expressed exosome-associated proteins. Finally, salivary exosomes isolated using these protocols can further be characterized for downstream analysis according to their c
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Robert L. Baker,Birgitte R. Mednickly, an immunoaffinity-based EV separation method followed by EV characterization, quantification, and normalization, as well as consecutive miRNA isolation and miRNA profiling by small RNA sequencing, are described.
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Protocol for Measuring Concentrations of Extracellular Vesicles in Human Blood Plasma with Flow Cytope specific EVs within a known diameter range and fluorescence range. More specifically, this protocol describes how to determine the concentration of CD61+ (Integrin beta-3, platelet marker), CD235a+ (Glycophorin A, erythrocyte marker), and CD45+ (leukocyte common antigen) EVs in human blood plasma
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