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Titlebook: Expression, Purification, and Structural Biology of Membrane Proteins; Camilo Perez,Timm Maier Book 2020 Springer Science+Business Media,

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Sarah C. Vigmostad,H.S. Udaykumar methods can easily be used to assess the thermostability and functionality of a GPCR sample exposed to different conditions, such as the use of various detergents, addition of lipids and ligands, making them valuable for obtaining an optimal sample quality for structural and functional studies.
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https://doi.org/10.1007/978-1-4419-6679-7e for enlarging nanobodies on their C-terminal end to generate “macrobodies,” without interfering with their original characteristics. These enlarged nanobodies extend the application as a chaperone in crystallography and can serve to increase the mass for small targets in single particle electron c
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Bone Density and Mechanical Property,nctional data on these proteins under defined conditions. To that end, different techniques have been developed to stabilize integral membrane proteins in native-like environments that allow both structural and biophysical investigations—amphipols, lipid bicelles, and lipid nanodiscs. In this chapte
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Geometric Analysis of Light Field Renderingamily of scramblases also function as nonselective ion channels. To better understand the physiological relevance of this channel function as well as its relationship to the scrambling activity of the TMEM16s we also describe in detail an in vitro flux assay to measure nonselective channel activity.
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