Titlebook: Expression, Purification, and Structural Biology of Membrane Proteins; Camilo Perez,Timm Maier Book 2020 Springer Science+Business Media,

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https://doi.org/10.1007/b101495f recombinant membrane proteins. Purification of homogeneous samples of membrane protein expressed in . is a significant bottleneck for researchers, and the protocol we present here for the overexpression and purification of membrane proteins in . will provide a solid basis to develop lab- and prote

Muffle

Image and Video-Based Artistic Stylisationrapeutic targets. The gram-positive bacterium . is an attractive option for heterologous membrane protein expression and purification thanks to advantageous characteristics such as mild proteolytic activity and small genome size. Vectors designed for gene transcription under the control of inducible

Retrieval

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CLAY

o identify the best-suited detergent, which on the one hand would solubilize large amounts of the target protein but on the other hand would sustain the protein’s activity. Here we describe the solubilization screen and subsequent activity assay we have optimized for the bacterial P-type ATPase KdpF

Urgency

Immunization

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