Titlebook: Experimental Models of Parkinson’s Disease; Yuzuru Imai Book 2021 The Editor(s) (if applicable) and The Author(s), under exclusive license

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Surfaces of Negative Curvature,hosphorylation, which functioned in the suppression of lysosomal enlargement as well as the promotion of the exocytic release of lysosomal cathepsins. In this chapter, we introduce two methods to analyze cellular functions of LRRK2 upon exposure to lysosomal overload stress in RAW264.7 cells.

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https://doi.org/10.1007/978-3-642-61779-9ne (MPTP), and rotenone, which inhibit the mitochondrial complex I, are widely used. Animal models of PD using these neurotoxins are also known as mitochondrial toxin models. Here this chapter describes the preparation of these mitochondrial toxin models.

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Geometry -Intuition and Conceptsdies. Recent studies on PD genes associated with lysosomal function suggest that GCase activity is decreased in cell models of PD and in neurons derived from PD patients. In this chapter, we describe a protocol to measure GCase activity in cultured cells.

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Differentiation of Midbrain Dopaminergic Neurons from Human iPS Cells induction of dopaminergic neurons from human iPS cells. Here, we describe an efficient method for differentiating human iPS cells into midbrain dopaminergic neurons. This protocol holds merit for obtaining a deeper understanding of the disease and for developing novel treatments.

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Monitoring PINK1-Parkin Signaling Using Dopaminergic Neurons from iPS Cells. Moreover, dopaminergic neurons could provide new findings not obtained in other cells. In this chapter, we will describe our method for monitoring PINK1-Parkin signaling using iPS cell-derived dopaminergic neurons.