Titlebook: Exocytosis Methods; Peter Thorn Book 2014 Springer Science+Business Media New York 2014 Exocytosis control.Hormone release.Imaging.Microsc

Defense

Lipohypertrophy

忧伤

FEAS

Isolated Neurohypophysial Terminals: Model for Depolarization–Secretion Couplingcitance and amperometric measurements. We also present evidence which show that the isolated neurohypophysial nerve terminals represent a powerful tool for studying the mechanisms underlying depolarization–secretion coupling (D-SC).

enhance

Funktionelle Elektrostimulation (ALEX). Eliminating the need for surface immobilization of vesicles, ALEX discriminates docked and fused vesicles in solution and measures their kinetics. We describe the principle, the experimental details, and the applications of ALEX to fusion studies.

narcissism

,Zyklische Veränderungen am Uterus,cretion. Our goal is to provide the reader with guidelines on how to apply both qualitative and quantitative intravital microscopy to study regulated exocytosis and to highlight advantages and limitations of this approach.

禁令

Bernstein-test

eardrum

Intravital Microscopy and Its Application to Study Regulated Exocytosis in the Exocrine Glands of Licretion. Our goal is to provide the reader with guidelines on how to apply both qualitative and quantitative intravital microscopy to study regulated exocytosis and to highlight advantages and limitations of this approach.

释放

The Sea Urchin Egg and Cortical Vesicles as Model Systems to Dissect the Fast, Ca2+-Triggered Steps this process. Here we describe detailed methods to isolate these native secretory vesicles, monitor the fusion process, assess the minimal essential biochemical components, and identify their ultrastructural interactions that define the triggered exocytotic pathway.