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Titlebook: Exocytosis Methods; Peter Thorn Book 2014 Springer Science+Business Media New York 2014 Exocytosis control.Hormone release.Imaging.Microsc

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书目名称Exocytosis Methods
编辑Peter Thorn
视频video
概述Includes cutting-edge techniques and methods for studying exocytosis in the brain.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the
丛书名称Neuromethods
图书封面Titlebook: Exocytosis Methods;  Peter Thorn Book 2014 Springer Science+Business Media New York 2014 Exocytosis control.Hormone release.Imaging.Microsc
描述.Bringing together techniques and methods currently being applied to the study of exocytosis, .Exocytosis Methods. collects chapters from experts in the field, examining this fundamental process essential to functions ranging from protein secretion to hormone release and neurotransmission.  The book begins with a section covering a range of techniques being applied to the study of single-vesicle fusion events, which are key in order to gain insight into the final steps of vesicle fusion.  The volume continues with several model systems that are being employed to unravel the complexities of exocytosis.  Written for the .Neuromethods. series, chapters included in this work present the kind of detail and vital implementation advice that leads to successful results.. .Practical and authoritative, .Exocytosis Methods. seeks to promote the advent of new methods in microscopy and the development of new preparations, which would doubtlessly lead to many new and exciting discoveries in this field..
出版日期Book 2014
关键词Exocytosis control; Hormone release; Imaging; Microscopy; Neuronal systems; Neurotransmission; Protein sec
版次1
doihttps://doi.org/10.1007/978-1-62703-676-4
isbn_softcover978-1-4939-6329-4
isbn_ebook978-1-62703-676-4Series ISSN 0893-2336 Series E-ISSN 1940-6045
issn_series 0893-2336
copyrightSpringer Science+Business Media New York 2014
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Funktionelle Pathologie in der Chirurgieated with perinatal lethality. More recent methods to study exocytosis specifically in the intact tissues include polar tracers and lipophilic dyes that can be used to directly visualize exocytosis with multiphoton excitation microscopy. A hallmark of the microscopic approach is that it offers posit
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0893-2336 e the advent of new methods in microscopy and the development of new preparations, which would doubtlessly lead to many new and exciting discoveries in this field..978-1-4939-6329-4978-1-62703-676-4Series ISSN 0893-2336 Series E-ISSN 1940-6045
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Imaging of Insulin Exocytosis from Pancreatic Beta Cells granules exist as large–dense core structures that are discernible by electron microscopy as an electron-dense interior surrounded by a clear region in a 300–350-nm intracellular membrane-delineated compartment (Greider et al., J Cell Biol 41:162–166, 1969; Lange, J Ultrastruct Res 46:301–307, 1974
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Rapid Analysis of Synaptic Vesicle Endocytosis in Synaptosomessured by the uptake of the fluorescent styryl dye FM4-64. The synaptosomes may be used fresh or frozen and stored in liquid nitrogen, with the latter option greatly expanding the utility of the assay, by reducing the animal numbers required and allowing experiments to be done “on demand.” The assay
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