Titlebook: Epitranscriptomics; Methods and Protocol Narendra Wajapeyee,Romi Gupta Book 2019 Springer Science+Business Media, LLC, part of Springer Nat

独行者

Visualization of , Long Noncoding RNA with a Fluorescent CRISPR/Cas9 System,PR/Cas9-based approach. This strategy is relatively simple approach to track . at different stages of cell differentiation, providing mechanistic insights into the initiation, maintenance, and establishment of X inactivation.

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novelty

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https://doi.org/10.1007/978-3-322-98482-1ofile m.A along the transcriptome. We present here a protocol for m.A crosslinking immunoprecipitation sequencing (m.A-CLIP-seq), which profiles m.A on mRNA at high resolution from as little as 1 μg of poly(A)-selected mRNA.

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Ewald Balcar,Stephen W. Loveseyn-reagent, as exemplified on a 110 nucleotide RNA obtained via in vitro transcription. The method exploits the fact that, under neutral buffer conditions (~pH 6.8), the 5′-phosphate carries the only mildly acidic proton in the RNA molecule, which allows for selective functionalization at that site using diazo reagents.

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Effects of Air Pollutants on Vegetation,bisulfite sequencing (RNA-BisSeq). Here we introduce an optimized RNA-BisSeq method by using ACT random hexamers to prime the reverse transcription of bisulfite-treated RNA samples to detect the m.C sites.

inspiration

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mendacity