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Titlebook: Epitranscriptomics; Methods and Protocol Narendra Wajapeyee,Romi Gupta Book 2019 Springer Science+Business Media, LLC, part of Springer Nat

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书目名称Epitranscriptomics
副标题Methods and Protocol
编辑Narendra Wajapeyee,Romi Gupta
视频videohttp://file.papertrans.cn/314/313386/313386.mp4
概述Includes cutting-edge methods and protocols.Provides step-by-step detail essential for reproducible results.Contains key notes and implementation advice from the experts
丛书名称Methods in Molecular Biology
图书封面Titlebook: Epitranscriptomics; Methods and Protocol Narendra Wajapeyee,Romi Gupta Book 2019 Springer Science+Business Media, LLC, part of Springer Nat
描述.This volume provides readers with the latest technologies to study changes in the epitranscriptome. The protocols described in this book explore both targeted and unbiased high-throughput analysis associated with post-transcriptional RNA modification. The chapters in this book also cover specific topics such as transcriptome-wide detection of 5-methylcytosine; HAMR; iRNA-2OM; genome-wide annotation of circRNAs; immune-northern blotting; and detection and quantification of pseudouridine in RNA. Written in the highly successful .Methods in Molecular Biology. series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls..Authoritative and comprehensive, .Epitranscriptomics: Methods and Protocols. is an important resource for both expert and novice scientists who are interested in learning more aboutthis field..
出版日期Book 2019
关键词Eukaryotic cells; yeast meiosis; single-cell RNA-sequencing; ESAT; dsRNAome; immune-northern blotting; pos
版次1
doihttps://doi.org/10.1007/978-1-4939-8808-2
isbn_ebook978-1-4939-8808-2Series ISSN 1064-3745 Series E-ISSN 1940-6029
issn_series 1064-3745
copyrightSpringer Science+Business Media, LLC, part of Springer Nature 2019
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Single-Molecule Analysis of RNA Dynamics in Living Cells Using Molecular Beacons,n length, function as key regulators of gene expression in cellular physiology and pathogenesis. Greater understanding of lincRNA activities, particularly in the context of subcellular localization and dynamic regulation at the single-molecule level, is expected to provide in-depth understanding of
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Visualization of , Long Noncoding RNA with a Fluorescent CRISPR/Cas9 System,chromosome in mammalian females. But the mechanism by which . localizes and spreads on the X chromosome and facilitates transcriptional silencing remains largely unknown. This limited understanding, at least in part, is due to the technical difficulties in the visualization and functional characteri
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,Direct Chemical Biotinylation of RNA 5′-Ends Using a Diazo Reagent,ng of in vitro transcribed RNA are scarce. Herein, we describe experimental details for direct labeling of the 5′-phosphate of RNA using a diazo biotin-reagent, as exemplified on a 110 nucleotide RNA obtained via in vitro transcription. The method exploits the fact that, under neutral buffer conditi
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Genome-Wide Annotation of circRNAs and Their Alternative Back-Splicing/Splicing with CIRCexplorer Pwide across different species. Interestingly, one gene locus can generate multiple circRNAs through alternative back-splicing and/or alternative splicing, thus expanding our understanding on the diversity and complexity of transcriptomes. Precise annotation of circRNAs with their alternative back-sp
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