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Titlebook: Epitope Mapping Protocols; Johan Rockberg,Johan Nilvebrant Book 2018Latest edition Springer Science+Business Media, LLC, part of Springer

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Epitope Mapping Protocols978-1-4939-7841-0Series ISSN 1064-3745 Series E-ISSN 1940-6029
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,Animal Models of Huntington’s Disease,or inspection of specific antibody-antigen interactions, even in the case of complex conformational epitopes. The method described here can be adapted for structure determination and epitope mapping of any antibody fragment to a simple or complex antigen.
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Introduction: Modernity for Animals?,ins on the surface of Gram positive bacteria, followed by domain-targeted mutagenesis and library analysis for the identification of key-residues by flow sorting and sequencing. Identified clones with reduced affinity are validated by single clone FACS and subsequent full-length expression in mammalian cells for validation.
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