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Titlebook: Epitope Mapping Protocols; Johan Rockberg,Johan Nilvebrant Book 2018Latest edition Springer Science+Business Media, LLC, part of Springer

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Animal Models of Central Neuropathic Pain,X-ray crystallography and NMR are considered the gold standard for providing precise information about these binding sites at atomic resolution. However, these are labor-intensive and require purified protein at high concentration. We have recently described [.] a rapid and reliable method that over
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Neurology of Speech and Languageng peptides were designed based upon a known amino acid sequence of the target protein, VapA (Virulence-associated Protein A) of the bacterium ., an important pulmonary pathogen in foals. The peptides synthesized as biotinylated peptides were coated directly onto micro titer plates which had been pr
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Anorexia-Like Wasting Syndromes in Pigscations. Hence, knowledge of the precise binding site (epitope) of antibodies on the target protein is one of the most important features for understanding its performance and determining its reliability in immunoassays. Here, we describe an updated protocol for high-resolution method for mapping ep
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Introduction: Modernity for Animals?,tanding method of action and for stratification of patients carrying the necessary epitope for desired drug efficacy, but also for capture assays under native conditions. Several epitope mapping methodologies have been described for this purpose, with the laborious X-ray crystallography method being
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Investigations of Goldfish Color Vision,ask. Planar antigen microarrays are very suitable tools to address this task and provide a powerful proteomics platform for the characterization of the binding selectivity of antibodies toward thousands of antigens in parallel. In this chapter, we describe our in-house developed procedures for the g
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